Influence of nonsteroidal anti-inflammatory drugs on progesterone production by cultured bovine luteal cells.

The objective of the present study was to determine the influence of nonsteroidal anti-inflammatory drugs (NSAIDs) representing different chemical groups on progesterone (P4) production by cultured bovine steroidogenic luteal cells. The cells were enzymatically isolated from corpora lutea collected on days 8-12 of the estrous cycle. After 24 h preincubation they were incubated for 24 h with medium only (control) or stimulated with bovine luteinizing hormone - LH (100 ng/ml; positive control) or increasing concentrations (10(-8) to 10(-4) M) of acetylsalicylic acid, indomethacin, ibuprofen, naproxen, piroxicam, phenylbutazone, dipyrone or nimesulide. Concentartions of P4 in the culture media were determined by enzyme immunoassay. LH significantly increased P4 secretion, while acetylsalicylic acid and indomethacin did not affect the production of this hormone. A significant increase in P4 secretion was observed after administration of dipyrone at all concentrations, piroxicam at concentrations of 10(-8), 10(-7) and 10(-5) M, phenylbutazone and naproxen at concentrations of 10(-7) and 10(-6) M and ibuprofen at concentrations of 10(-5) and 10(-4) M. Nimesulide did not affect P4 production at concentrations of 10(-8) - 10(-5) M, while at a concentration of 10(-4) M it inhibited P4 secretion. The results obtained indicate that NSAIDs may change the production of P4 in bovine luteal cells, however, these changes are dependent on the substance used.